Human tumour drug sensitivity testing in vitro

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Human Tumour Drug Sensitivity Testing In Vitro: Techniques and Clinical ApplicationsAuthor: K C Calman. Predictive drug testing on human tumor cells in order to define the appropriate chemotherapy will remain imperative as long as the anticancer agents available are.

Get this from a library. Human tumour drug sensitivity testing in vitro: techniques and clinical applications. [P P Dendy; Bridget T Hill;]. Human Tumour Drug Sensitivity Testing In Vitro: Techniques and Clinical Applications By K C Calman Topics: Book ReviewsAuthor: K C Calman.

The plating efficiency of tumour cells obtained from spheroids was always greater than for the cells obtained from the dissociated tumour of origin and was in Cited by: Although a number of chemosensitivity tests have been proposed since the advent of modern anticancer chemotherapy, interest has been renewed by the possibility of cloning human tumor cells on agar plates, with a view to testing drug activity on cells with high pro­ liferation capacity.

INTRODUCTION In Hamburger and Salmon [1] introduced an assay for the clonogenic cells of human tumours which has since been widely exploited in an attempt to put cancer chemotherapy on a rational footing by allowing the oncologist to choose the drugs which he will use to treat an individual patient on the basis of in vitro chemosensitivity tests of that patient's tumour by: 2.

The technical limitations of the test are presented, and it is concluded that with the present status of the test, it is only suited as a preclinical test system.

It is suggested that in vitro sensitivity testing of drugs in a panel of cell lines established from patients with small cell carcinoma of the lung might help to identify drugs that could alter the clinical course of the by: 4.

A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow. The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late s as an in vitro drug Cited by: In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids.

In so doing, they combine the latest technological developments with strategic outlooks, such as novel cell and tissue systems for drug screening and testing, as well as emerging in vitro culture technologies. Equally importantly, the book does not.

The Importance of Cancer Cell Lines as in vitro Models in Cancer Methylome Analysis and Anticancer Drugs Testing tumour. This sample is limited in amount and the genetic manipulation is almost impossible [3].

The xenografts models (nude mice) are used for testing the tumorigenicity and metastatic ability of cancer cell lines. Summary. Background: Tumor cell drug sensitivity is an important determinant of chemotherapy measurement in vitro would aid in therapy individualization and new drug development.

Materials and methods: The fluorometric microculture cytotoxicity assay (FMCA), based on production by viable cells of fluorescent fluorescein after 3 days of culture, was used for cytotoxic drug Cited by: While testing of all solid tumours is not possible with present-day techniques, webelieve thatthe employmentofin vitro sensitivity testing as aroutineproceduremay bepossible inthefutureifasuitable systemgivingcorrelation betweenin vitro andin vivosensitivity can be developed.

Since the advent of cytotoxic chemotherapy in theCited by: 3. A human tumor cloning assay was used to analyze drug sensitivity profiles for 99 pairs of tumor samples taken simultaneously from the same patient. These pairs included two areas of the same primary (12 pairs), primary tumor versus metastasis (29 pairs), and metastasis versus metastasis (45 Cited by: Drug sensitivity testing utilizing preclinical disease models such as cancer cell lines is an important and widely used tool for drug development.

Importantly, when combined with molecular data such as gene copy number variation or somatic coding mutations, associations between drug sensitivity and molecular data can be used to develop markers to guide patient therapies.

Friedman HM, Glaubiger DL: Assessment of in vitro drug sensitivity of human tumor cells using [3 H]-thymidine incorporation in a modified human tumor stem cell assay. Cancer Res –, Cancer Res –, Cited by: 1. Chemosensitivity Testing – What It Is and What It Isn’t July 1, By Robert A.

Nagourney, MD Several weeks ago I was consulted by a young man regarding the management of his heavily pre-treated, widely metastatic rectal cancer.

Effect of Leukocyte Interferons on Cell Proliferation of Human Tumors in Vitro.- Drug Combination Testing with In Vitro Clonal Cultures.- Neutralization of cis-Dichlorodiammineplatinum II and Nitrogen Mustard by Thiols.- Usefulness of the Human Tumor Colony Forming Assay for New Drug.

1. Determining the drug sensitivity of tumor cells, based on the results selecting the possibly most effective drugs and/or drug combinations for the therapy. The result of the in vitro drug sensitivity of cancer cells can be used as a prognostic factor.

2. Ranking cancer drugs. To test the device, the researchers implanted it in mice that had been grafted with human prostate, breast, and melanoma tumors. These tumors are known to have varying sensitivity to different cancer drugs, and the MIT team’s results corresponded to those previously seen differences.Cell culture-based in vitro drug sensitivity tests may be able to predict the sensitivity of cancer cells to various molecular target drugs under development for future clinical application.

SDI performed on clinical samples showed growth inhibition in patients with EGFR by: 5.